application-variables

I am writing a program recording service calls and treatment done. We have a number of users who open and close calls and I want to show at all times the total number of calls opened today and the total number closed today and the difference between them. I thought of doing it with an application variable. I have to reset these variables to 0 every day. Where would I do that? I thought in the Global.asax but in which event could that be done? The application is running all the time so I suppose Application_Start wouldn’t be appropriate. So where? Thank you.